The Agilent Seahorse XF Flex analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates in a 24-well format. It offers optimized workflows and dedicated consumables to enable real-time metabolic analysis with 3D study models, such as organoids and tissue materials.

Key Features / Highlights

• A flexible 24-well system that allows real-time metabolic analysis with improved sensitivity and consistency, using two-dimensional (2D) and three-dimensional (3D) study models.
• Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP production rates.
• Four-port injection system with automated mixing feature for assessing immediate cellular, tissue organoid responses to substrates, inhibitors, and other compounds in real time.
• Broad operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C.
• Automated data quality check in data analysis software to flag abnormal assay behavior.

Technical Specifications

• Analyzer Application – Cellular Energy Metabolism Studies – Phenotyping, 2D Cultures
• Assay Running Volume – 600-1000 µL/well
• Assay Wells – 22

Applications

• Cell Analysis
• Discover the power of metabolism
• Immune Metabolism
• Cancer Metabolism

Link to the Original Product Page: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers/seahorse-xf-flex-analyzer-9318021